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5S rRNA互作蛋白组研究揭示5S rRNA参与mRNA剪接调控

5S rRNA interactome reveals that 5S rRNA is involved in mRNA splicing regulation

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【作者】 周祥明杨兵赖巧李乐董军廖建友朱爽

【Author】 ZHOU Xiangming;YANG Bing;LAI Qiao;LI Le;DONG Jun;LIAO Jianyou;ZHU Shuang;School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University;Sun Yat-sen Memorial Hospital, Sun Yat-sen University;

【通讯作者】 朱爽;

【机构】 广东药科大学生命科学与生物制药学院中山大学孙逸仙纪念医院

【摘要】 为系统揭示5S rRNA调控细胞进程的新功能和分子机制,合成了生物素标记探针,利用分子杂交技术特异捕获5S rRBP,并结合高灵敏度质谱技术和生物信息学分析共鉴定出162个5S rRNA互作蛋白。基于ConsensusPathDB和STRING数据库获取蛋白复合体富集信息和构建蛋白与蛋白互作网络,利用GO数据库获取互作蛋白的功能注释,利用KEGG数据库进行通路富集分析。分析结果显示5S rRNA除了与翻译相关蛋白发生互作外,还与大量来自microRNA加工通路、mRNA剪接通路和细胞信号调控通路蛋白有互作。这说明5S rRNA除了具有蛋白质翻译调控功能外,还参与mRNA剪接等过程的调控,为5S rRNA功能和调控机制研究指明了一个新的方向。

【Abstract】 This study aims to systematically reveal the new function and molecular mechanism of 5 S rRNA regulating cell process. The biotin-labeled probe was synthesized, and the 5 S rRBP was obtained by molecular hybridization. 162 5 S rRNA interacting proteins was identified by high sensitivity mass spectrometry and bioinformatics analysis. Protein complex-based gene sets analysis was based on ConsensusPathDB. Protein-protein interaction network was constructed basing on STRING database. The GO database was used for acquiring functional annotations of interactive proteins. KEGG database was used for pathway enrichment analysis. The analysis showed that in addition to interacting with translation-related proteins, 5 S rRNA also interacts with a large number of proteins from microRNA processing pathways, mRNA splicing pathways and signal pathway regulation pathways, etc. The result indicates that 5 S rRNA not only have the function of protein translation regulation, but also participate in the regulation of mRNA splicing and other processes. This study points out a new direction for the study of 5 S rRNA function and regulatory mechanism.

【关键词】 5S rRNAChIRP-MS互作蛋白组mRNA剪接
【Key words】 5S rRNAChIRP-MSinteractomemRNA splicing
【基金】 广东省恶性肿瘤表观遗传与基因调控重点实验室基金(2017B030314026);广东省科技计划项目(2017A030313066);广东省自然科学基金(2016A020221037);国家自然科学基金(41271263)
  • 【文献出处】 中山大学学报(自然科学版) ,Acta Scientiarum Naturalium Universitatis Sunyatseni , 编辑部邮箱 ,2019年03期
  • 【分类号】Q75
  • 【下载频次】24
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